Úplné zobrazení záznamu

Toto je statický export z katalogu ze dne 30.06.2018. Zobrazit aktuální podobu v katalogu.

Bibliografická citace

.
0 (hodnocen0 x )
BK
AKVIZICE
Cambridge University Press, 2018.


ISBN 978-1316614761
001463119
CONTENTS // Foreword to the Eighth Edition Preface // List of Contributors // 0 Tables and Resources // 1 Biochemical and Molecular Biological Methods in Life Sciences Studies // Samuel Clokie and Andreas Hofmann // 1.1 From Biochemistry and Molecular Biology to the Life Sciences // 1.2 The Education of Life Scientists // 1.3 Aims of Life Science Studies // 1.4 Personal Qualities and Scientific Conduct // 1.5 Suggestions for Further Reading // 2 Basic Principles // Parisa Amani and Andreas Hofmann // 2.1 Biologically Important Molecules // 2.2 The Importance of Structure // 2.3 Parameters of Biological Samples // 2.4 Measurement of the pH: The pH Electrode // 2.5 Buffers // 2.6 Ionisation Properties of Amino Acids // 2.7 Quantitative Biochemical Measurements // 2.8 Experiment Design and Research Conduct // 2.9 Suggestions for Further Reading // 3 Cell Culture Techniques // Anwar R. Baydoun // 3.1 Introduction // 3.2 The Cell Culture Laboratory and Equipment // 3.3 Safety Considerations in Cell Culture // 3.4 Aseptic Techniques and Good Cell Culture Practice // 3.5 Types of Animal Cells, Characteristics and Maintenance in Culture // 3.6 Stem Cell Culture // 3.7 Bacterial Cell Culture // 3.8 Potential Use of Cell Cultures // page xiii // XV // xvii // xxi // 1 // 1 // 2 // 3 // 5 // 7 // 8 // 8 // 9 // 18 // 24 // 26 // 30 // 32 // 33 38 // 40 // 40 // 41 46 46 51 61 67 70 // v // vi Contents // 3.9 Acknowledgements 71 // 3.10 Suggestions for Further Reading 71 // 4 Recombinant
DNA Techniques and Molecular Cloning 73 // Ralph Raplcy // 4.1 Introduction 73 // 4.2 Structure of Nucleic Acids 74 // 4.3 Genes and Genome Complexity 80 // 4.4 Location and Packaging of Nucleic Acids 83 // 4.5 Functions of Nucleic Acids 85 // 4.6 The Manipulation of Nucleic Acids: Basic Tools and Techniques 96 // 4.7 Isolation and Separation of Nucleic Acids 98 // 4.8 Automated Analysis of Nucleic Acid Fragments 103 // 4.9 Molecular Analysis of Nucleic Acid Sequences 104 // 4.10 The Polymerase Chain Reaction (PCR) 110 // 4.11 Constructing Gene Libraries 118 // 4.12 Cloning Vectors 128 // 4.13 Hybridisation and Gene Probes 145 // 4.14 Screening Gene Libraries 146 // 4.15 Applications of Gene Cloning 148 // 4.16 Expression of Foreign Genes 153 // 4.17 Analysing Genes and Gene Expression 157 // 4.18 Analysing Genetic Mutations and Polymorphisms 167 // 4.19 Molecular Biotechnology and Applications 173 // 4.20 Pharmacogenomics 175 // 4.21 Suggestions for Further Reading 176 // 5 Preparative Protein Biochemistry 179 // Samuel Clokie // 5.1 Introduction 179 // 5.2 Determination of Protein Concentrations 180 // 5.3 Engineering Proteins for Purification 184 // 5.4 Producing Recombinant Protein 188 // 5.5 Cell-Disruption Methods 191 // 5.6 Preliminary Purification Steps 195 // 5.7 Principles of Liquid Chromatography 196 // 5.8 Chromatographic Methods for Protein Purification 206 // 5.9 Other Methods of Protein Purification 210 // 5.10 Monitoring Protein Purification 213 // 5.11 Storage
217 // 5.12 Suggestions for Further Reading 218 // 6 Electrophoretic Techniques 219 // Ralph Rapley // 6.1 General Principles 219 // Contents // vii // 6.2 Support Media and Buffers 223 // 6.3 Electrophoresis of Proteins 226 // 6.4 Electrophoresis of Nucleic Acids 240 // 6.5 Capillary Electrophoresis 246 // 6.6 Microchip Electrophoresis 250 // 6.7 Suggestions for Further Reading 252 // 7 Immunochemical Techniques 253 // Katja Fischer // 7.1 Introduction 253 // 7.2 Antibody Preparation 261 // 7.3 Immunoassay Formats 271 // 7.4 Immuno Microscopy 277 // 7.5 Lateral Flow Devices 278 // 7.6 Epitope Mapping 279 // 7.7 Immunoblotting 279 // 7.8 Fluorescence-Activated Cell Sorting (FACS) 280 // 7.9 Cell and Tissue Staining Techniques 281 // 7.10 Immunocapture Polymerase Chain Reaction (PCR) 281 // 7.11 Immunoaffinity Chromatography 282 // 7.12 Antibody-Based Biosensors 282 // 7.13 Luminex® Technology 283 // 7.14 Therapeutic Antibodies 283 // 7.15 Suggestions for Further Reading 285 // 8 Flow Cytometry 287 // John Grainger and Joanne Konkel // 8.1 Introduction 287 // 8.2 Instrumentation 289 // 8.3 Fluorescence-Activated Cell Sorting (FACS) 294 // 8.4 Fluorescence Labels 294 // 8.5 Practical Considerations 298 // 8.6 Applications 305 // 8.7 Suggestions for Further Reading 312 // 9 Radioisotope Techniques 313 // Robert J. Slater // 9.1 Why Use a Radioisotope? 313 // 9.2 The Nature of Radioactivity 314 // 9.3 Detection and Measurement of Radioactivity 322 // 9.4 Other Practical Aspects of
Counting Radioactivity // and Analysis of Data 334 // 9.5 Safety Aspects 34O // 9.6 Suggestions for Further Reading 344 // viii Contents // 10 Principles of Clinical Biochemistry 346 // GUI Rumsby // 10.1 Principles of Clinical Biochemical Analysis 346 // 10.2 Clinical Measurements and Quality Control 350 // 10.3 Examples of Biochemical Aids to Clinical Diagnosis 361 // 10.4 Suggestions for Further Reading 380 // 11 Microscopy 381 // Stephen W. Paddoek // 11.1 Introduction 381 // 11.2 The Light Microscope 384 // 11.3 Optical Sectioning 396 // 11.4 Imaging Live Cells and Tissues 403 // 11.5 Measuring Cellular Dynamics 407 // 11.6 The Electron Microscope 411 // 11.7 Image Management 417 // 11.8 Suggestions for Further Reading 420 // 12 Centrifugation and Ultracentrifugation 424 // Kay Ohlendieek and Stephen E. Harding // 12.1 Introduction 424 // 12.2 Basic Principles of Sedimentation 425 // 12.3 Types, Care and Safety Aspects of Centrifuges 430 // 12.4 Preparative Centrifugation 438 // 12.5 Analytical Ultracentrifugation 446 // 12.6 Suggestions for Further Reading 452 // 13 Spectroscopic Techniques 454 // Anne Simon and Andreas Hofmann // 13.1 Introduction 454 // 13.2 Ultraviolet and Visible Light Spectroscopy 460 // 13.3 Circular Dichroism Spectroscopy 471 // 13.4 Infrared and Raman Spectroscopy 476 // 13.5 Fluorescence Spectroscopy 479 // 13.6 Luminometry 492 // 13.7 Atomic Spectroscopy 494 // 13.8 Rapid Mixing Techniques for Kinetics 497 // 13.9 Suggestions for Further Reading
498 // 14 Basic Techniques Probing Molecular Structure and Interactions 500 // Anne Simon and Joanne Maedonald // 14.1 Introduction 500 // 14.2 Isothermal Titration Calorimetry 501 // 14.3 Techniques to Investigate the Three-Dimensional Structure 502 // 14.4 Switch Techniques 521 // Contents // ix // 14.5 Solid-Phase Binding Techniques with Washing Steps 524 // 14.6 Solid-Phase Binding Techniques Combined with Flow 526 // 14.7 Suggestions for Further Reading 532 // 15 Mass Spectrometric Techniques 535 // Sonja Hess and James L MacRae // 15.1 Introduction 535 // 15.2 Ionisation 537 // 15.3 Mass Analysers 549 // 15.4 Detectors 556 // 15.5 Other Components 557 // 15.6 Suggestions for Further Reading 557 // 16 Fundamentals of Bioinformatics 559 // Cinzia Cantaeessi and Anna V. Protasio // 16.1 Introduction 559 // 16.2 Biological Databases 560 // 16.3 Biological Data Formats 563 // 16.4 Sequence Alignment and Tools 569 // 16.5 Annotation of Predicted Peptides 576 // 16.6 Principles of Phylogenetics 584 // 16.7 Suggestions for Further Reading 596 // 17 Fundamentals of Chemoinformatics 599 // Paul Taylor // 17.1 Introduction 599 // 17.2 Computer Representations of Chemical Structure 602 // 17.3 Calculation of Compound Properties 614 // 17.4 Molecular Mechanics 616 // 17.5 Databases 626 // 17.6 Suggestions for Further Reading 627 // 18 The Python Programming Language 631 // Tim J. Stevens // 18.1 Introduction 631 // 18.2 Getting Started 632 // 18.3 Examples 661 // 18.4 Suggestions for
Further Reading 676 // 19 Data Analysis 677 // Jean-Baptiste Cazier // 19.1 Introduction 677 // 19.2 Data Representations 679 // 19.3 Data Analysis 702 // 19.4 Conclusion 730 // 19.5 Suggestions for Further Reading 73? // Contents // 20 Fundamentals of Genome Sequencing and Annotation 732 // Paši ?. Korhonen and Robin B. Gasser // 20.1 Introduction 732 // 20.2 Genomic Sequencing 733 // 20.3 Assembly of Genomic Information 737 // 20.4 Prediction of Genes 742 // 20.5 Functional Annotation 745 // 20.6 Post-Genomic Analyses 752 // 20.7 Factors Affecting the Sequencing, Annotation and Assembly // of Eukaryotic Genomes, and Subsequent Analyses 754 // 20.8 Concluding Remarks 755 // 20.9 Suggestions For Further Reading 755 // 21 Fundamentals of Proteomics 758 // Sonja Hess and Michael Weiss // 21.1 Introduction: From Edman Sequencing to Mass Spectrometry 758 // 21.2 Digestion 759 // 21.3 Tandem Mass Spectrometry 759 // 21.4 The Importance of Isotopes for Finding the Charge State of a Peptide 765 // 21.5 Sample Preparation and Handling 767 // 21.6 Post-Translational Modification of Proteins 769 // 21.7 Analysing Protein Complexes 772 // 21.8 Computing and Database Analysis 776 // 21.9 Suggestions for Further Reading 777 // 22 Fundamentals of Metabolomics 779 // James /. MacRae // 22.1 Introduction: What is Metabolomics? 779 // 22.2 Sample Preparation 780 // 22.3 Data Acquisition 785 // 22.4 Untargeted and Targeted Metabolomics 787 // 22.5 Chemometrics and Data Analysis 797 // 22.6 Further
Metabolomics Techniques and Terminology 802 // 22.7 Suggestions for Further Reading 807 // 23 Enzymes and Receptors 809 // Megan Cross and Andreas Hofmann // 23.1 Definition and Classification of Enzymes 809 // 23.2 Enzyme Kinetics 813 // 23.3 Analytical Methods to Investigate Enzyme Kinetics 831 // 23.4 Molecular Mechanisms of Enzymes 838 // 23.5 Regulation of Enzyme Activity 840 // 23.6 Receptors 844 // 23.7 Characterisation of Receptor-Ligand Binding 845 // 23.8 Suggestions for Further Reading 862 // Contents // xi // 24 Drug Discovery and Development 864 // David Camp // 24.1 Introduction 864 // 24.2 Molecular Libraries and Drug-Discovery Strategies 865 // 24.3 Assembling a Molecular Library 881 // 24.4 Compound Management 887 // 24.5 Screening Strategies Used in Hit Discovery 889 // 24.6 Active-to-Hit Phase 895 // 24.7 Hit-to-Lead Phase 895 // 24.8 ADMET 896 // 24.9 Lead Optimisation 909 // 24.10 Suggestions for Further Reading 909 // Index // 906

Zvolte formát: Standardní formát Katalogizační záznam Zkrácený záznam S textovými návěštími S kódy polí MARC